Review



rnase iii  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
      Buy from Supplier

    Structured Review

    New England Biolabs rnase iii
    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or <t>RNase</t> <t>III</t> (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.
    Rnase Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase iii/product/New England Biolabs
    Average 96 stars, based on 297 article reviews
    rnase iii - by Bioz Stars, 2026-02
    96/100 stars

    Images

    1) Product Images from "Template nicking suppresses promoter-independent antisense transcription in IVT via R-loop-mediated strand displacement"

    Article Title: Template nicking suppresses promoter-independent antisense transcription in IVT via R-loop-mediated strand displacement

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaf1536

    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or RNase III (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.
    Figure Legend Snippet: Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or RNase III (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.

    Techniques Used: Agarose Gel Electrophoresis, Synthesized, Dot Blot



    Similar Products

    rnase iii  (New England Biolabs)
    96
    New England Biolabs rnase iii
    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or <t>RNase</t> <t>III</t> (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.
    Rnase Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase iii/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    rnase iii - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    10x shortcut reaction buffer  (New England Biolabs)
    96
    New England Biolabs 10x shortcut reaction buffer
    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or <t>RNase</t> <t>III</t> (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.
    10x Shortcut Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x shortcut reaction buffer/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    10x shortcut reaction buffer - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    shortcut rnase iii  (New England Biolabs)
    96
    New England Biolabs shortcut rnase iii
    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or <t>RNase</t> <t>III</t> (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.
    Shortcut Rnase Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shortcut rnase iii/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    shortcut rnase iii - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    10x mncl 2  (New England Biolabs)
    96
    New England Biolabs 10x mncl 2
    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or <t>RNase</t> <t>III</t> (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.
    10x Mncl 2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10x mncl 2/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    10x mncl 2 - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    shortcut rnaseiii  (New England Biolabs)
    96
    New England Biolabs shortcut rnaseiii
    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or <t>RNase</t> <t>III</t> (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.
    Shortcut Rnaseiii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shortcut rnaseiii/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    shortcut rnaseiii - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    shortcut rnase iii new england biolabs cat  (New England Biolabs)
    96
    New England Biolabs shortcut rnase iii new england biolabs cat
    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or <t>RNase</t> <t>III</t> (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.
    Shortcut Rnase Iii New England Biolabs Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/shortcut rnase iii new england biolabs cat/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    shortcut rnase iii new england biolabs cat - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or RNase III (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.

    Journal: Nucleic Acids Research

    Article Title: Template nicking suppresses promoter-independent antisense transcription in IVT via R-loop-mediated strand displacement

    doi: 10.1093/nar/gkaf1536

    Figure Lengend Snippet: Promoter-independent antisense transcription contributes to dsRNA. ( A ) 1% agarose gel electrophoresis analysis of eGFP RNA synthesized by T7 RNA polymerase and treated with increasing concentrations of S1 nuclease (0.04, 0.08, 0.16, and 0.24 U) or RNase III (0.002, 0.004, 0.008, and 0.016 U). ( B ) 1% agarose gel and J2 antibody-based dot blot analysis of RNA transcribed from four DNA templates: (i) ssDNA containing only the T7 promoter region (80 nt), (ii) single-stranded template strand (904 nt), (iii) partially duplexed DNA with 80 bp encompassing the T7 promoter and a single-stranded downstream region, and (iv) fully duplexed DNA. For both analyses, 200 ng of each IVT RNA was loaded. As controls, 200 ng of ssRNA and 20 ng of dsRNA markers were included on the agarose gel, and 200 ng of ssRNA and 10 ng of dsRNA were spotted on the dot blot to confirm J2 antibody specificity. Total RNA was quantified using the RiboGreen assay. Values for total RNA and dsRNA were normalized to the yield obtained from the fully duplexed dsDNA template. Statistical comparisons were performed using one-way ANOVA with Šídák’s multiple comparisons test; **** P < .0001.

    Article Snippet: To assess RNA structure, 400 ng of IVT RNA was digested with S1 nuclease (Thermo Fisher Scientific, #EN0321) or RNase III (New England Biolabs, #M0245S).

    Techniques: Agarose Gel Electrophoresis, Synthesized, Dot Blot